Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse

<p>Abstract</p> <p>Background</p> <p>The equine periodontium provides tooth support and lifelong tooth eruption on a remarkable scale. These functions require continuous tissue remodeling. It is assumed that multipotent mesenchymal stromal cells (MSC) reside in the periodontal ligament (PDL) and play a crucial role in regulating physiological periodontal tissue regeneration. The aim of this study was to isolate and characterize equine periodontal MSC.</p> <p>Tissue samples were obtained from four healthy horses. Primary cell populations were har-vested and cultured from the gingiva, from three horizontal levels of the PDL (apical, midtooth and subgingival) and for comparison purposes from the subcutis (masseteric region). Colony-forming cells were grown on uncoated culture dishes and typical <it>in vitro </it>characteristics of non-human MSC, i.e. self-renewal capacity, population doubling time, expression of stemness markers and trilineage differentiation were analyzed.</p> <p>Results</p> <p>Colony-forming cell populations from all locations showed expression of the stemness markers CD90 and CD105. In vitro self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFU-F) assays. CFU-efficiency was highest in cell populations from the apical and from the mid-tooth PDL. Population doubling time was highest in subcutaneous cells. All investigated cell populations possessed trilineage differentiation potential into osteogenic, adipogenic and chondrogenic lineages.</p> <p>Conclusions</p> <p>Due to the demonstrated in vitro characteristics cells were referred to as equine subcutaneous MSC (eSc-MSC), equine gingival MSC (eG-MSC) and equine periodontal MSC (eP-MSC). According to different PDL levels, eP-MSC were further specified as eP-MSC from the apical PDL (eP-MSCap), eP-MSC from the mid-tooth PDL (eP-MSCm) and eP-MSC from the subgingival PDL (eP-MSCsg). Considering current concepts of cell-based regenerative therapies in horses, eP-MSC might be promising candidates for future clinical applications in equine orthopedic and periodontal diseases.</p

Пам'яті Миколи Чумаченка

14 жовтня на 87-ому році життя відійшов у вічність видатний учений-економіст, доктор економічних наук, академік Національної академії наук України, заслужений діяч науки і техніки України, лауреат Державної премії України в галузі науки і техніки, лауреат премій АН УРСР ім. О.Г. Шліхтера та НАН України ім. М.І. Тугана-Барановського, почесний директор Інституту економіки промисловості НАН України, ветеран Великої Вітчизняної війни, почесний громадянин міста Донецька голова редакційної колегії журналу Схід з економіки Микола Григорович Чумаченко

Психокоррекция коммуникативной формы сексуальной дезадаптации у мужчин с органическим расстройством личности и изменением полового предпочтения

Представлена разработанная автором система психотерапии коммуникативной формы сексуальной дезадаптации супругов при девиантном сексуальном поведении мужчин с органическим расстройством личности. Предложенная система апробирована с хорошим терапевтическим эффектом у дезадаптированных супружеских пар, в которых у мужей наблюдалось изменение полового предпочтения.The original system of psychotherapy for communicative sexual dysadaptation of spouses at deviant sexual behavior of men with organic personality disorders is presented. The proposed system was tested with a good therapeutic effect in dysadapted married couples, in which the husband had changes of sexual preference

Chronic Somatic Comorbidity and Excess Mortality Due to Natural Causes in Persons with Schizophrenia or Bipolar Affective Disorder

Background: Suicide and death by accidents in persons with schizophrenia and bipolar disorder are common, but excess mortality from natural death accounts for even more years of life lost. The impact of somatic comorbidity, however, often is not duly considered in analyses and explanations of excess mortality in patients with psychotic disorders. Objective/Methods: This study investigates and evaluates the impact of 19 severe chronic diseases on excess mortality due to diseases and medical conditions (natural death) in individuals with psychotic disorders compared with the general population using a population-based cohort study in Denmark. Incidence/mortality rate ratios of admission/mortality were calculated using survival analysis. Results: Cohort members with psychotic disorders had higher incidence rates of hospital contacts for almost all of the 19 disorders than the general population. The mortality rate ratio (MRR) of natural death was 7.10 (95 % CI 6.45, 7.81) for schizophrenic men, decreasing to 4.64 (95 % CI 4.21, 5.10) after adjustment for the somatic disorders. The same pattern existed in women and in both genders with bipolar disorder. Highest MRRs were observed for psychotic patients without hospital admissions with the investigated somatic disorders. Conclusion: Chronic somatic diseases accounted for half of the excess mortality in patients with schizophrenia or bipolar disorder. Chronic disorders investigated in this paper seem to be under-treated or under-detected among such patients

Sur quelques travertins perivolcaniques et sur leur florule de diatomées

Diese Notiz beschreibt eimge kieselartige oder kalkartige « travertins » von der volkanish Kreis Mont-Dore und Intrusions Kaminen (pipes) von « les Causses ». Der paleontologisches Studium des Diatomee-Bevölkerung zeigt das, fur die Speziesmenge Vermehrung, eine calciumarme Umgebung nicht fruchtbar ist.This note describes some siliceous or calcareous travertines from the volcanic country of « Mont-Dore » and the breccia pipes of the « Causses ». The paleontological investigation on diatoma populations shows that a low percentage of calcium during the formation prevents the proliferation of numerous species.L'objet de la présente note est l'étude de quelques travertins ,siliceux ou calcaires, en relation avec le volcanisme du Mont-Dore et les cheminées intrusives (pipes) des Causses. L'analyse paléontologique des populations de diatomées a permis de remarquer notamment que la pauvreté du milieu en calcium empêche la prolifération d'un grand nombre d'espèces.Fournier-Gasse Françoise, Causse Christiane, Marval Viviane, Brousse R. Sur quelques travertins perivolcaniques et sur leur florule de diatomées. In: Bulletin de l'Association française pour l'étude du quaternaire, vol. 3, n°3, 1966. pp. 202-207